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Article: Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree

Article from:
In Vitro Cellular & Developmental Biology
Article date:
November 1, 2002
Author:
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SUMMARY

Somatic embryos were obtained from a 60-yr-old Quercus suber L. tree. Leaf explants were cultivated on Murashige and Skoog medium with 30 gl^sup -1^ sucrose, 3 gl^sup -1^ gelrite, pH adjusted to 5.8, and different growth regulator combinations. Callus induction took place at 24 +/- 1 deg C in the dark during the first 3 wk. After 3 mo. calluses that showed embryogenic structures were transferred to the same medium without growth regulators. Somatic embryogenesis was only observed in calluses induced on E3 medium (supplemented with 4.5 (mu)M 2,4-dichlorophenoxyacetic acid and 9.0 (mu)M zeatin). On average, 7.5% of the initial explants formed embryogenic calluses in this medium. ...

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