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Exposing ferrets to cigarette smoke and a pharmacological dose of (beta)-carotene supplementation enhance in vitro retinoic acid catabolism in lungs via induction of cytochrome...

Exposing Ferrets to Cigarette Smoke and a Pharmacological Dose of beta-Carotene Supplementation Enhance In Vitro Retinoic Acid Catabolism in Lungs via Induction of Cytochrome P450 Enzymes1,2

ABSTRACT In our previous studies, we found lower levels of retinoic acid (RA) in the lungs of ferrets exposed to cigarette smoke and/or a pharmacological dose of beta-carotene. To determine whether this is involved in excessive catabolism of RA via cytochrome P450 (CYP) induction, we carried out in vitro incubations of RA with the lung microsomal fractions of ferrets with or without CYP inhibitors and antibodies against CYP. The polar metabolites (4-oxo-RA and 18-hydroxy-RA) of RA metabolism after the ...

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