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Article: Pollination-, development-, and auxin-specific regulation of gibberellin 3beta-hydroxylase gene expression in pea fruit and seeds
- Article from:
- Plant Physiology
- Article date:
- March 1, 2003
- Author:
CopyrightCopyright American Society of Plant Physiologists Mar 2003. Provided by ProQuest LLC. (Hide copyright information)
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Pollination-, Development-, and Auxin-Specific Regulation of Gibberellin 3,beta-Hydroxylase Gene Expression in Pea Fruit and Seeds1
To understand further how pollination, seeds, auxin (4-chloroindole-3-acetic acid [4-Cl-IAA]), and gibberellins (GAs) regulate GA biosynthesis in pea (Pisum sativum) fruit, we studied expression of the gene PsGA3oxl that codes for the enzyme that converts GA^sub 20^ to biologically active GAl using real-time reverse transcription-polymerase chain reaction analysis. PsGA3oxl mRNA levels were minimally detectable in prepollinated pericarps and ovules (-2 d after anthesis [DAA]), increased dramatically after pollination (0 DAA), then decreased by 1 DAA. Seed ...