Magazine article from our research archive:
|
|
Muscle-derived CD45-SCA-1 + C-KIT- progenitor cells give rise to skeletal muscle myotubes in vitro
- Article from:
-
In Vitro Cellular & Developmental Biology
- Article date:
-
October 1, 2002
- Author:
-
;
;
;
;
|
Copyright informationCopyright Society for In Vitro Biology Oct 2002. Provided by ProQuest LLC. (Hide copyright information)
|
(Received 26 July 2002; accepted 19 December 2002)
SUMMARY
A stem cell population isolated from murine skeletal muscle has recently been shown to differentiate into hematopoietic cells after transplantation in vivo. In the present study, we tested the hypothesis that this cell population would also, under appropriate culture conditions, differentiate into skeletal muscle cells in vitro. Lower-extremity skeletal muscle tissue isolated from 3- to 4-wk-old mice was dissected free from bone and vessels, enzymalically digested, and flow cytometrically sorted to yield CD45^sup -^Sca-1^sup +^c-Kit^sup -^ (S+) cells. These cells were further sorted into CD34^sup +^ and CD34^sup -^ fractions and ...
Related newspaper, magazine, and journal articles:
|
Calcium-Dependent Facilitation and Graded Deactivation of Store-Operated...
Biophysical Journal;
July 1, 2004 ;
700+ words
......whereas SR luminal Ca^sup 2+^ can deactivate SOCE in the fetal skeletal muscle. This cooperative...voltage-gated Ca^sup 2+^ channel located on the PM of skeletal muscle has slow activation...voltage-gated Ca^sup 2+^ entry should exist in fetal skeletal muscle to ...
|
|
|
Sustained overexpression of IGF-1 prevents age-dependent decrease in...
Biophysical Journal;
March 1, 2002 ;
700+ words
......intracellular Ca^sup 2+^ in skeletal muscle fibers. To this...peak cytosolic Ca^sup 2+^, with a subsequent decrease in skeletal muscle force. Based on...that IGF-1 enhances skeletal muscle charge movement, [^sup 3^H]PN200-110 binding...
|
|
|
Calmodulin modulates initiation but not termination of spontaneous Ca(2+)...
Biophysical Journal;
August 1, 2003 ;
700+ words
......the local Ca^sup 2+^ release...permeabilized frog skeletal muscle fibers. Fluorescently...termination of Ca^sup 2+^ release from the SR in skeletal muscle. In muscle RYR...setting. Ca^sup 2+^ sparks have...measured in frog skeletal muscle upon fiber ...
|
|
|
Multiple regions of RyR1 mediate functional and structural interactions...
Biophysical Journal;
December 1, 2002 ;
700+ words
......DHPRs) and RyRs within Ca^sup 2+^ release units (CRUs). In skeletal muscle this interaction is bidirectional...receptor) to release Ca^sup 2+^ in the absence of...alpha^sub 1C^DHPR Ca^sup 2+^ current is important...coupling, indicated by Ca^sup 2+^ transients in the...movement ...
|
|
|
Ca(2+)-dependent interaction between FKBP12 and calcineurin regulates...
Biophysical Journal;
November 1, 2002 ;
700+ words
......Calcineurin is a Ca^sup 2+^ and calmodulin-depende...derived from mouse skeletal muscle. Coimmunoprecipitation...exhibits a strong Ca^sup 2+^ dependence...role in the Ca^sup 2+^ signaling of...INTRODUCTION In skeletal muscle, coupling of electrical...intracellular ...
|
|
|
Differential effects of voltage-dependent inactivation and local...
Biophysical Journal;
July 1, 2003 ;
700+ words
......In voltage-clamped frog skeletal muscle fibers, Ca^sup 2+^ release rises rapidly...selectively, disable its Ca^sup 2+^-dependent or its voltage-operat...two-stage control of Ca^sup 2+^ release in muscle, whereby Ca^sup 2+^ released through multiple...junctions to secondarily open ...
|
|
|
Transient Loss of Voltage Control of Ca^sup 2+^ Release in the Presence of...
Biophysical Journal;
September 15, 2006 ;
700+ words
......MCa into mouse skeletal muscle fibers and measured...sustained phase of Ca^sup 2+^ elevation...e-c) coupling of skeletal muscle. The contraction of a skeletal muscle fiber is rapidly...cytoplasmic [Ca^sup 2+^] that activates...MCa into adult skeletal ...
|
|
|
Short-Term Regulation of Excitation-Contraction Coupling by the [beta]^sub...
Biophysical Journal;
December 1, 2005 ;
700+ words
......subunit of the skeletal muscle voltage-gated Ca^sup 2+^ channel plays...contraction in skeletal muscle (for reviews...permeant to Ca^sup 2+^, generating...effect on Ca^sup 2+^ release induced...potentials in adult skeletal muscle fibers. Preliminary...
|
|
Taking skeletal muscle to heart.
Science News;
November 19, 1988 ;
700+ words
...Taking Skeletal Muscle to Heart Would you have your songs...increasingly are finding that skeletal muscle can be wrapped around a damaged...valuable assistance. Indeed, while skeletal muscle may never match the heart for...
|
|
Simple quantification of skeletal muscle glucose utilization by static...
The Journal of Nuclear Medicine;
October 1, 2003 ;
700+ words
......resistance. Key Words: skeletal muscle; glucose utilization...insulin resistance; ^sup 18^F-FDG; PET...performed to evaluate skeletal muscle insulin resistance by ^sup 18^F-FDG PET...the kinetics of ^sup 18^F-FDG in human skeletal muscle (27), like that...
|
|
Bioimpedance analysis: Potential for measuring lower limb skeletal muscle...
JPEN, Journal of Parenteral and Enteral Nutrition;
March 1, 1999 ;
700+ words
......dependent on lower limb skeletal muscle mass. At present, both...evaluating lower limb skeletal muscle mass as a component...when height squared (H^sup 2^) is used instead...limb length squared (L^sup 2^) in multiple regression...lower limb fat and skeletal muscle were ...
|
|
|
Differential Interaction of Cardiac, Skeletal Muscle, and Yeast...
Biophysical Journal;
February 15, 2006 ;
700+ words
......tropomyosin to yeast actin, we mutated S^sup 235^ to C and labeled the actin with pyrene maleimide at both C^sup 235^ and the normally reactive C^sup 374^. Saturating cardiac tropomyosin...actin but no change in WT actin C^sup 374^ probe fluorescence. Skeletal muscle tropomyosin ...
|
|
|
Regulation of the calcium release channel from skeletal muscle by suramin...
Biophysical Journal;
March 1, 2003 ;
700+ words
...ABSTRACT Activation of skeletal muscle ryanodine receptors...cytoplasmic [Ca^sup 2+^] and the bilayer...such as ATP, Ca^sup 2+^, Mg^sup 2+^, and pH, is...sequenced: RyR-1 (skeletal muscle), RyR-2 (cardiac...RyR-1 from rabbit skeletal muscle in both SR ...
|
See all results.
Or, try our
Advanced Search.
|