|
|
Article: Matrix synthesis by bladder smooth muscle cells is modulated by stretch frequency
- Article from:
- In Vitro Cellular & Developmental Biology
- Article date:
- March 1, 2003
- Author:
CopyrightCopyright Society for In Vitro Biology Mar/Apr 2003. Provided by ProQuest LLC. (Hide copyright information)
|
SUMMARY
The bladder is a physically active organ that undergoes periodic stretching as a part of its normal function. To determine the role that stretching or mechanical deformation may play in altering the synthetic phenotype ol bladder wall cells, a series of experiments were carried out to quantify several extracellular matrix (ECM) messenger ribonucleic acids (mRNAs) and their corresponding protein levels after mechanical challenge. Bladder smooth muscle cells were grown on distensible membranes in an apparatus that can reliably and reproducibly subject cells to well-characterized periods of mechanical stretching. For this study, cultured bovine bladder cells were subjected to cyclic ...