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Article: Establishment and characterization of 13 cell lines from a green turtle (chelonia mydas) with fibropapillomas
- Article from:
- In Vitro Cellular & Developmental Biology
- Article date:
- July 1, 1999
- Author:
CopyrightCopyright Society for In Vitro Biology Jul/Aug 1999. Provided by ProQuest LLC. (Hide copyright information)
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SUMMARY
Thirteen cell lines were established and characterized from brain, kidney, lung, spleen, heart, liver, gall bladder, urinary bladder, pancreas, testis, skin, and periorbital and tumor tissues of an immature male green turtle (Chelonia mydas) with fibropapillomas. Cell lines were optimally maintained at 30 deg C in RPMI 1640 medium supplemented with 10% fetal bovine serum. Propagation of the turtle cell lines was serum dependent, and plating efficiencies ranged from 13 to 37%. The cell lines, which have been subcultivated more than 20 times, had a doubling time of approximately 30 to 36 h. When tested for their sensitivity to several fish viruses, most of the cell lines were ...