Article: Accumulation of Short n-Chain Ethyl Esters by Esterases of Lactic Acid Bacteria Under Conditions Simulating Ripening Parmesan Cheese

ABSTRACT

EstA from Lactobacillus helueticus CNRZ32 (LbhEstA), EstB, and EstC from Lactobacillus casei LILA, and EstA from Lactococcus lactis MG1363 (Lcl-EstA) were evaluated for their ability to accumulate esters in a model system simulating Parmesan cheese ripening conditions (1O0C, 2 to 3% NaCl, pH 5.4 to 5.5, a^sub w^ = 0.850 to 0.925) using Capalase K from kid goat as a positive control. all of the LAB esterases and Capalase K mediated the accumulation of esters in the model system in an enzyme specific manner, which was influenced by a^sub w^ and selectivity for fatty acid chain-length. In general, enzyme mediated accumulation of ethyl esters was higher at aw values of 0.850 and 0.900 ...

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