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Article: Kinetics of ^sup 99m^Tc-Labeled Interleukin-8 in Experimental Inflammation and Infection
- Article from:
- The Journal of Nuclear Medicine
- Article date:
- September 1, 2003
- Author:
CopyrightCopyright Society of Nuclear Medicine Sep 2003. Provided by ProQuest LLC. (Hide copyright information)
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The cytokine interleukin-8 (IL-8) binds with high affinity to the CXCR1 and CXCR2 receptors on neutrophils. In previous studies, we showed that ^sup 99m^Tc-IL-8 could rapidly and effectively delineate foci of infection and inflammation in rabbit models of intramuscular infection, colitis, and osteomyelitis. Here, the in vivo kinetics and pharmacodynamics of ^sup 99m^Tc-IL-8 are studied in detail. A derivative of hydrazinonicotinamide (HYNIC) was used as a bifunctional coupling agent to label the protein with ^sup 99m^Tc. Methods: To address specificity of uptake of ^sup 99m^Tc-IL-8 in the abscess, uptake in turpentine-induced abscesses in neutropenic rabbits was compared with uptake in ...