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Article: Ascorbic Acid Does Not Increase the Oxidative Stress Induced by Dietary Iron in C3H Mice1
- Article from:
- The Journal of Nutrition
- Article date:
- February 1, 2004
- Author:
CopyrightCopyright American Institute of Nutrition Feb 2004. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT Iron is a potent prooxidant that can induce lipid peroxidation. Ascorbic acid, a potent antioxidant, has prooxidant effects in the presence of iron in vitro. We investigated whether ascorbic acid and iron co-supplementation in ascorbic acid-sufficient mice increases hepatic oxidative stress. C3H/He mice were fed diets supplemented with iron to 100 mg/kg diet or 300 mg/kg diet with or without ascorbic acid (15 g/kg diet) for 3 wk. Liver iron concentration, malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST), glutathione peroxidase (GPx), Superoxide dismutase (SOD), and catalase (CAT) were measured. High dietary iron increased liver iron concentrations slightly ...