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Article: [alpha]-Tocopherol and Ascorbic Acid Decrease the Production of [beta]-Apo-carotenals and Increase the Formation of Retinoids from [beta]-Carotene in the Lung Tissues of Cigarette Smoke-Exposed Ferrets In Vitro1
- Article from:
- The Journal of Nutrition
- Article date:
- February 1, 2004
- Author:
CopyrightCopyright American Institute of Nutrition Feb 2004. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT Previously, we found that exposing ferrets to cigarette smoke enhanced oxidative excentric cleavage of [beta]-carotene. In the present study, we examined whether [alpha]-tocopherol, ascorbic acid, or the two combined can prevent smoke-altered [beta]-carotene metabolism. In vitro incubation of [beta]-carotene (10 µmol/L) with lung postnuclear fractions from ferrets exposed to cigarette smoke was carried out in the absence or presence of [alpha]-tocopherol (50 µmol/L), ascorbic acid (10 or 50 µmol/L), or both vitamins to evaluate their effects on the production of [beta]-apo-carotenals and retinoids from [beta]-carotene. The oxidative cleavage metabolites of [beta]-carotene, ...