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Article: Topology of the Maize Mixed Linkage (1[arrow right]3),(1[arrow right]4)-[beta]-D-Glucan Synthase at the Golgi Membrane1

Article from:
Plant Physiology
Article date:
February 1, 2004
Author:
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Mixed-linkage (1[arrow right]3),(1[arrow right]4)-[beta]-D-glucan is a plant cell wall polysaccharide composed of cellotriosyl and cellotetraosyl units, with decreasingly smaller amounts of cellopentosyl, cellohexosyl, and higher cellodextrin units, each connected by single (1[arrow right]3)-[beta]-linkages. (1[arrow right]3),(1[arrow right]4)-[beta]-Glucan is synthesized in vitro with isolated maize (Zea mays) Golgi membranes and UDP-[^sup 14^C]D-glucose. The (1[arrow right]3),(1[arrow right]4)-[beta]-glucan synthase is sensitive to proteinase K digestion, indicating that part of the catalytic domain is exposed to the cytoplasmic face of the Golgi membrane. The d1etergent ...

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