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Article: Time course of changes in absorption of mactomolecule through the nasal mucosa after antigen challenge in guinea pig model of allergic rhinitis
- Article from:
- The Annals of Otology, Rhinology & Laryngology
- Article date:
- December 1, 2000
- Author:
CopyrightCopyright Annals Publishing Company Dec 2000. Provided by ProQuest LLC. (Hide copyright information)
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The time course of changes in absorption of horseradish peroxidase (HRP) through the nasal mucosa after antigen challenge was evaluated in a guinea pig model of allergic rhinitis immunized with ovalbumin. Before and at 5 minutes, 4 hours, and 24 hours after nasal antigen challenge, both nasal cavities were filled with 5% HRP solution for 30 minutes, and blood was obtained to measure serum HRP levels by enzyme-linked immunosorbent assay. In immunized animals, the serum HRP levels were 2.3 times higher than those of normal controls (p < .05) before antigen challenge, which was performed 7 days after a series of nasal antigenic sensitizations. At 5 to 35 minutes after antigen challenge, the ...