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Article: Isolation and characterization of the genes up-regulated in isolated neurons by aged garlic extract (AGE)
- Article from:
- The Journal of Nutrition
- Article date:
- March 1, 2001
- Author:
CopyrightCopyright American Institute of Nutrition Mar 2001. Provided by ProQuest LLC. (Hide copyright information)
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Isolation and Characterization of the Genes Up-Regulated in Isolated Neurons by Aged Garlic Extract (AGE)1
ABSTRACT Aged garlic extract (AGE) produces neurotrophic effects on cultured fetal rat hippocampal neurons. These studies examined the molecular events triggered by AGE that might account for a suppression of neuronal cell death. Genes differentially expressed by the addition of AGE in primary cultured hippocampal neurons isolated from fetal rat brain were screened using mRNA differential display. Four cDNA clones were significantly enhanced at their transcriptional level; they were designated as #24, #110, #153 and #155. Quantitative reverse transcription-- polymerase chain reaction ...