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Monitoring Neural Activity In Vivo
- Article from:
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The Scientist
- Article date:
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September 27, 2004
- Author:
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Copyright informationCopyright The Scientist, Inc. Sep 27, 2004. Provided by ProQuest LLC. (Hide copyright information)
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New fosGFP transgenic mice light up as they learn
Scientists at Carnegie Mellon University in Pittsburgh have created a transgenic mouse that will allow researchers to visualize patterns of activity directly in individual neurons in vivo.1 To create the animals, Alison Earth and colleagues coupled the c-fos promoter, which is typically activated during neural activity, to a green fluorescent protein (GFP) marker. "By coupling c-fos activation to the expression of GFP, I could now see cells that were specifically implicated by particular stimuli, and specifically stimuli in vivo," Barth explains.
Barth was interested in locating individual neurons engaged in a behavior (such as learning) or ...
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