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Monitoring Neural Activity In Vivo

New fosGFP transgenic mice light up as they learn

Scientists at Carnegie Mellon University in Pittsburgh have created a transgenic mouse that will allow researchers to visualize patterns of activity directly in individual neurons in vivo.1 To create the animals, Alison Earth and colleagues coupled the c-fos promoter, which is typically activated during neural activity, to a green fluorescent protein (GFP) marker. "By coupling c-fos activation to the expression of GFP, I could now see cells that were specifically implicated by particular stimuli, and specifically stimuli in vivo," Barth explains.

Barth was interested in locating individual neurons engaged in a behavior (such as learning) or ...

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