|
|
Article: DNA Gyrase Is Involved in Chloroplast Nucleoid Partitioning
- Article from:
- Plant Cell
- Article date:
- October 1, 2004
- Author:
CopyrightCopyright American Society of Plant Physiologists Oct 2004. Provided by ProQuest LLC. (Hide copyright information)
|
DNA gyrase, which catalyzes topological transformation of DNA, plays an essential role in replication and transcription in prokaryotes. Virus-induced gene silencing of NbGyrA or NbGyrB, which putatively encode DNA gyrase subunits A and B, respectively, resulted in leaf yellowing phenotypes in Nicotiana benthamiana. NbGyrA and NbGyrB complemented the gyrA and gyrB temperature-sensitive mutations of Escherichia coli, respectively, which indicates that the plant and bacterial subunits are functionally similar. NbGyrA and NbGyrB were targeted to both chloroplasts and mitochondria, and depletion of these subunits affected both organelles by reducing chloroplast numbers and inducing morphological ...