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Article: Ebola virus glycoprotein demonstrates differential cellular localization in infected cell types of nonhuman primates and guinea pigs
- Article from:
- Archives of Pathology & Laboratory Medicine
- Article date:
- May 1, 2001
- Author:
CopyrightCopyright College of American Pathologists May 2001. Provided by ProQuest LLC. (Hide copyright information)
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* Background.-In vitro studies have previously shown that Ebola virus glycoprotein (GP) is rapidly processed and largely released from infected cells, whereas other viral proteins, such as VP40, accumulate within cells.
Objective.-To determine infected cell types in which Ebola virus GP and VP40, individually, localize in vivo.
Methods.-Immunohistochemistry and in situ hybridization using GP- and VP40-specific antibodies and genetic probes were used to analyze archived tissues of experimentally infected nonhuman primates and guinea pigs and Vero E6 and 293 cells infected in vitro.
Results.-The GP antigen was consistently present in hepatocytes, adrenal cortical cells, fibroblasts, ...