Article: ³-Glutamyl Transpeptidase Inhibition Suppresses Milk Protein Synthesis in Isolated Ovine Mammary Cells

ABSTRACT

The membrane spanning enzyme ³-glutamyl transpeptidase (³-GT; EC 2.3.2.2) catalyses the breakdown of the tripeptide glutathione and uses free amino acids (AA) to form ³-glutamyl transpeptidase (GT) AA that become transported into cells and converted back into free AA. ³-Glutamyl transpeptidase activity has been shown to be important for mammary AA uptake in rodent systems, and while ³-GT activity is high in lactating bovine mammary tissue, the role of this enzyme in milk protein synthesis of the ruminant has not been denned. The present study shows that ³-GT activity in the ovine mammary gland, like that of rodents, increases during pregnancy and peaks early in lactation. ...

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