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Route of Tracer Administration Does Not Affect Ileal Endogenous Nitrogen Recovery Measured with the ^sup 15^N-Isotope Dilution Technique in Pigs Fed Rapidly Digestible Diets1,2
- Article from:
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The Journal of Nutrition
- Article date:
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November 1, 2004
- Author:
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Copyright informationCopyright American Institute of Nutrition Nov 2004. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
The ^sup 15^N-isotope dilution technique (^sup 15^N-IDT), with either pulse-dose oral administration or continuous i.v. administration of [^sup 15^N]-L-leucine (carotid artery), both at 5 mg/(kg body weight . d), was used to measure ileal (postvalve T-cecum cannula) endogenous nitrogen recovery (ENR) in pigs (9 ± 0.6 kg). Diets were cornstarch, enzyme-hydrolyzed casein with no (control) or high (4%) content of quebracho extract (Schinopsis spp.) rich in condensed tannins. Blood was sampled from a catheter in the external jugular vein. Mean plasma ^sup 15^N-enrichment at d 8-10 was higher (P = 0.0009) after i.v. than after oral administration [0.0356 vs. 0.0379 atom% excess (APE)]. ...
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