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Article: ANCYMIDOL-ENHANCED HYPERHYDRIC MALFORMATION IN RELATION TO GIBBERELLIN AND OXIDATIVE STRESS IN LIQUID-CULTURED NARCISSUS LEAVES
- Article from:
- In Vitro Cellular & Developmental Biology
- Article date:
- November 1, 2004
- Author:
CopyrightCopyright Society for In Vitro Biology Nov/Dec 2004. Provided by ProQuest LLC. (Hide copyright information)
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SUMMARY
The growth retardant ancymidol inhibited gibberellin biosynthesis and enhanced hyperhydric malformation of Narcissus leaf sections cultured in liquid medium. Superoxide dismutase activities were examined by spectrophotometry and native polyacrylamide gel analysis, and gibberellin and hydrogen peroxide levels were determined spectrophotometrically in either hyperhydric or non-hyperhydric leaf sections. In ancymidol-treated hyperhydric leaf sections, Superoxide dismutase activity and hydrogen peroxide levels were higher during the initial culture period, when hyperhydric malformation occurred, than in control untreated leaf sections. At a later stage, when the meristematic centers ...