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Article: HLA-DR2 subtypes & immune responses in pulmonary tuberculosis
- Article from:
- Indian Journal of Medical Research
- Article date:
- April 1, 2001
- Author:
CopyrightCopyright Indian Council of Medical Research Apr 2001. Provided by ProQuest LLC. (Hide copyright information)
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Background & objectives: HLA-DR2 has been shown to be associated with the susceptibility to pulmonary tuberculosis and altered antibody and lymphocyte response in pulmonary tuberculosis. In the present study, the influence of DR2 subtypes on antibody titre and lymphocyte response to Mycobacterium tuberculosis culture filtrate antigens (10 pg/ml) was studied in 22 patients with active pulmonary TB (ATB), 50 inactive (cured) TB (ITB) patients and 36 healthy control subjects.
Methods: HLA-DR2 gene was amplified by polymerase chain reaction (PCR) and dot-blotted. Genotyping of DRBI*1501, *1502, *1503, *1601 and *1602 was carried out using sequence specific oligonucleotide probes (SSOPs) ...