Out and In: Simplifying Membrane Protein Studies by AFM

Biophysical Journal;  November 1, 2006 ;     700+ words ......structure-function relationship of membrane proteins in their native environment...only [approximate]30% are membrane proteins, they constitute >50...targets. This is because membrane proteins are virtually involved in...

The Protein-Tethered Lipid Bilayer: A Novel Mimic of the Biological...

Biophysical Journal;  November 1, 2004 ;     700+ words ......for the functional incorporation of membrane proteins is introduced. The incorporated protein...which histidine-tagged (His-tagged) membrane proteins are able to bind in a reversible...be applicable to all recombinant membrane proteins bearing a terminal His-tag. In particular...

MEMBRANE PROTEIN 'FACTORY' MAY LEAD TO NEW DRUG TREATMENTS

US Fed News Service, Including US State News;  June 2, 2006 ;     689 words ......factory that enables the study of membrane proteins. These proteins are challenging to...percent of drug targets. Studies of membrane proteins could lead to new and improved pharmaceutical...addictions and cystic fibrosis. Membrane proteins perform essential processes in the...

Comparison of helix interactions in membrane and soluble (alpha)-bundle...

Biophysical Journal;  May 1, 2002 ;     700+ words ......folding, stability, and function of membrane proteins. Here, two independent and complementary...tightly packed interfacial residues in membrane proteins are Gly, Ala, and Ser, and that helices...contact plots. We find that helices in membrane proteins exhibit a broader distribution ...

Anionic Phospholipid Interactions with the Potassium Channel KcsA:...

Biophysical Journal;  February 1, 2006 ;     700+ words ......revealed that in terms of lipid-protein interactions PA > PG [much greater...lipid interactions on a membrane protein surface. INTRODUCTION Membrane proteins are of considerable biophysical...biological perspective, membrane proteins play key roles in a ...

On the Accuracy of Homology Modeling and Sequence Alignment Methods...

Biophysical Journal;  July 15, 2006 ;     700+ words ......water-soluble proteins are appropriate for membrane proteins as well. To this end we present an...strategies for homology modeling of membrane proteins and introduce a benchmark data set...identity and structural similarity in membrane proteins. This analysis indicates that ...

Free Energy of Membrane Protein Unfolding Derived from Single-Molecule...

Biophysical Journal;  August 1, 2007 ;     700+ words ......the unfolding free energy of the membrane proteins bacteriorhodopsin (BR), halorhodopsin...variety of single- and multispan membrane proteins can be tackled in mechanical unfolding...elucidating energetic properties of membrane proteins with the resolution of individual...

UNIVERSITY OF MEDICINE & DENTISTRY OF NEW JERSEY BIOCHEMISTRY PROFESSOR...

US Fed News Service, Including US State News;  September 8, 2008 ;     567 words ......develop technology that determines membrane proteins' structure. Inouye's was one of...that determines the structure of membrane proteins. "The structure and functional study of membrane proteins is one of the most challenging...

A New Method for the Reconstitution of Membrane Proteins into Giant...

Biophysical Journal;  July 1, 2004 ;     700+ words ......method for the reconstitution of membrane proteins into giant unilamellar vesicles...reconstitution of two radically different membrane proteins, the sarcoplasmic reticulum Ca^sup...detergent-mediated reconstitution of solubilized membrane proteins into proteoliposomes of 0.1-0.2 m...

The sensitivity of saturation transfer electron paramagnetic resonance...

Biophysical Journal;  December 1, 2001 ;     700+ words ......global rotational motion of intrinsic membrane proteins that can interact with the cytoskeleton...configuration where the intrinsic membrane proteins are present at remarkably high local...fluid environment, the intrinsic membrane proteins that traverse the lipid bilayer can...

Scientists Devise Method to Study Membrane Proteins, One Key to Drug...

Ascribe Higher Education News Service;  April 8, 2004 ;     500 words ......the lipid membrane of cells. Such membrane proteins make up about 30 percent of all proteins...struggling to devise a method to keep membrane proteins stable enough for long-term structural...are effective because they work on membrane proteins, but our basic knowledge about these...

MediChem Life Sciences Adds Three Cutting-Edge Protein Scientists to Drug...

PR Newswire;  December 21, 2000 ;     700+ words ......coupled receptors (GPCRs) and other membrane proteins, including ion channels. -- Dr. Behnke...advanced technology for crystallizing membrane proteins. -- Dr. Nollert also has extensive...D., MediChem's president and CEO. Membrane proteins are the 'Holy Grail' of structural...

Helical Packing Patterns in Membrane and Soluble Proteins

Biophysical Journal;  December 1, 2004 ;     700+ words ......of interacting pairs of helices in membrane proteins of known structure was constructed...membrane-protein data set. Most helix pairs in membrane proteins are found to have a significant number...distributions that mirror those observed in membrane proteins. The larger average ...

High-Resolution AFM of Membrane Proteins Directly Incorporated at High...

Biophysical Journal;  November 1, 2006 ;     700+ words ......heterologous expression and purification of membrane proteins represent major limitations for their...bacterial photosynthetic multi-subunit membrane proteins. Height measurements by atomic force...two-dimensional crystallization of membrane proteins for the structural analysis by ...

German Inventors Develop Membrane Proteins Separation Method

US Fed News Service, Including US State News;  February 17, 2007 ;     323 words ......for electrophoretically separating membrane proteins. According to the U.S. Patent &...proteins extending through the membrane (membrane proteins) is a prerequisite for a complete...is to electrophoretically separate membrane proteins in one or two dimensions. To this...