|
|
Article: Technical Note: Molecular Typing of Corynebacterium bovis Isolates by Pulsed-Field Gel Electrophoresis
- Article from:
- Journal of Dairy Science
- Article date:
- May 1, 2005
- Author:
CopyrightCopyright American Dairy Science Association May 2005. Provided by ProQuest LLC. (Hide copyright information)
|
ABSTRACT
A typing method for Corynebacterium bovis based on pulsed-field gel electrophoresis (PFGE) is described and applied to type 162 strains isolated from mastitic milk from 57 Friesian dairy herds in northern Spain. Isolates were identified to the species level using the API-Coryne system and a polymerase chain reaction-restriction fragment length polymorphism of the 16S rDNA amplicon. Cultures in logarithmic phase were harvested, mixed with agarose, and treated with lisozyme and proteinase K to release intact genomic DNA that was digested wiihXbal and submitted to PFGE. Seven different pulsotypes were identified; none of them were similar to that of the reference strain (ATCC 7715). ...