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Article: Immunoquantitative PCR for Prion Protein Detection in Sporadic Creutzfeldt-Jakob Disease
- Article from:
- Clinical Chemistry
- Article date:
- September 1, 2005
- Author:
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Copyright informationCopyright American Association for Clinical Chemistry Sep 2005. Provided by ProQuest LLC. (Hide copyright information)
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Background: The most common human prion disorder is Creutzfeldt-Jakob disease (CJD); it includes sporadic, familial, iatrogenic, and variant subtypes. Diagnostic tests aim at detection with the highest specificity of very small deposits of abnormal prion protein (PrP).
Methods: We used immunoquantitative PCR (iqPCR) to detect proteinase K-resistant PrP (PrP^sup Res^) in tissue from the middle frontal gyrus of 7 patients with sporadic CJD and 7 non-CJD cases. We compared iqPCR with routine optimized ELISA, Western blotting, and immunohistochemical analyses.
Results: The 4 methods showed similar 100% sensitivity and specificity for the diagnosis of CJD. Along with high specificity, however, ...
Related newspaper, magazine, and journal articles:
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Article: There is no cure or test.(News)
The Mirror (London, England);
October 31, 2002 ;
72 words
...SARAH Shadbolt's husband Edward died from sporadic CJD, the same form of the disease at the centre of the Middlesbrough health scare. Unlike variant CJD, sporadic CJD usually incubates slowly, affecting those in their 50s and 60s. Mrs Shadbolt...
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