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Characterization and Spatial Distribution of Aphanomyces in Sugarbeet Fields

ABSTRACT

To study phenotypes and spatial distribution of Aphanomyces in sugarbeet fields with histories of root rot, 80 soil cores were collected 1-m apart along a transect in one field each in Minnesota (MN), North Dakota (ND), and Texas (TX). Aphanomyces was isolated from 52, 84, and 49% of the soil cores collected in MN, ND, and TX, respectively. Randomly amplified polymorphic DNA (RAPD) analysis was completed on 41, 41, and 39 isolates from MN, ND, and TX, respectively, and A. euteiches as an outgroup. A single polymorphic (1400 bp) RAPD product was specific to 56% of the Texas isolates. Oogonia of isolates from sugarbeet had a mean diameter of 23.2 µm compared to two isolates of A, ...

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