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Article: Identification and Quantification of Glycerolipids in Cotton Fibers: Reconciliation with Metabolic Pathway Predictions from DNA Databases
- Article from:
- Lipids
- Article date:
- August 1, 2005
- Author:
CopyrightCopyright AOCS Press Aug 2005. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT: The lipid profiles of cotton fiber cells were determined from total lipid extracts of elongating and maturing cotton fiber cells to see whether the membrane lipid composition changed during the phases of rapid cell elongation or secondary cell wall thickening. Total FA content was highest or increased during elongation and was lower or decreased thereafter, likely reflecting the assembly of the expanding cell membranes during elongation and the shift to membrane maintenance (and increase in secondary cell wall content) in maturing fibers. Analysis of lipid extracts by electrospray ionization and tandem MS (ESI-MS/MS) revealed that in elongating fiber cells (7-10 d post-anthesis), ...