Article: NH^sub 4^^sup +^ Currents across the Peribacteroid Membrane of Soybean. Macroscopic and Microscopic Properties, Inhibition by Mg^sup 2+^, and Temperature Dependence Indicate a SubpicoSiemens Channel Finely Regulated by Divalent Cations1

The control of ammonium (NH^sub 4^^sup +^) transport is critical in preventing futile cycles of NH^sub 4^^sup +^/ammonia transport. An unusual nonselective cation channel with subpicoSiemens single-channel conductance permeable to NH^sub 4^^sup +^ had previously been identified in the peribacteroid membrane (PBM) of symbiosomes from soybean (Glycine max) nodules. Here, we investigate the proposed channel mechanism and its control by luminal magnesium. Currents carried by NH^sub 4^^sup +^ were measured in inside-out PBM patches by patch clamp. NH^sub 4^^sup +^ transport corresponding to the physiological direction of net transfer showed time-dependent activation and associated ...

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