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Article: MODELING THE AIRWAY EPITHELIUM IN ALLERGIC ASTHMA: INTERLEUKIN-13-INDUCED EFFECTS IN DIFFERENTIATED MURINE TRACHEAL EPITHELIAL CELLS
- Article from:
- In Vitro Cellular & Developmental Biology
- Article date:
- July 1, 2005
- Author:
CopyrightCopyright Society for In Vitro Biology Jul/Aug 2005. Provided by ProQuest LLC. (Hide copyright information)
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SUMMARY
Mucous cells of the airway epithelium play a crucial role in the pathogenesis of human inflammatory airway diseases. Therefore, it is of importance to complement in vivo studies that use murine models of allergic asthma with in vitro mechanistic studies that use murine airway epithelial cells, including mucus-containing cells. In this study, we report the development and characterization of an in vitro culture system for primary murine tracheal epithelial (MTE) cells comprising ciliated cells and a substantial number of mucous cells. The increase in mucous cell number over that observed in the native murine airway, or in previously described murine cultures, creates a culture ...