Article: Intracellular and Extracellular Concentrations of Na^sup +^ Modulate Mg^sup 2+^ Transport in Rat Ventricular Myocytes

ABSTRACT

Apparent free cytoplasmic concentrations of Mg^sup 2+^ ([Mg^sup 2+^]^sub i^) and Na+ ([Na+]^sub i^) were estimated in rat ventricular myocytes using fluorescent indicators, furaptra (mag-fura-2) for Mg^sup 2+^ and sodium-binding benzofuran isophthalate for Na+, at 25°C in Ca^sup 2+^-free conditions. Analysis included corrections for the influence of Na+ on furaptra fluorescence found in vitro and in vivo. The myocytes were loaded with Mg^sup 2+^ in a solution containing 24 mM Mg^sup 2+^ either in the presence of 106 mM Na+ plus 1 mM ouabain (Na+ loading) or in the presence of only 1.6 mM Na+ to deplete the cells of Na+ (Na+ depletion). The initial rate of decrease in [Mg^sup ...

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