|
|
Article: Intracellular and Extracellular Concentrations of Na^sup +^ Modulate Mg^sup 2+^ Transport in Rat Ventricular Myocytes
- Article from:
- Biophysical Journal
- Article date:
- November 1, 2005
- Author:
CopyrightCopyright Biophysical Society Nov 2005. Provided by ProQuest LLC. (Hide copyright information)
|
ABSTRACT
Apparent free cytoplasmic concentrations of Mg^sup 2+^ ([Mg^sup 2+^]^sub i^) and Na+ ([Na+]^sub i^) were estimated in rat ventricular myocytes using fluorescent indicators, furaptra (mag-fura-2) for Mg^sup 2+^ and sodium-binding benzofuran isophthalate for Na+, at 25°C in Ca^sup 2+^-free conditions. Analysis included corrections for the influence of Na+ on furaptra fluorescence found in vitro and in vivo. The myocytes were loaded with Mg^sup 2+^ in a solution containing 24 mM Mg^sup 2+^ either in the presence of 106 mM Na+ plus 1 mM ouabain (Na+ loading) or in the presence of only 1.6 mM Na+ to deplete the cells of Na+ (Na+ depletion). The initial rate of decrease in [Mg^sup ...