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Article: Relationship of protein synthesis to mRNA levels in the liver of chicks under various nutritional conditions
- Article from:
- The Journal of Nutrition
- Article date:
- June 1, 1996
- Author:
CopyrightCopyright American Institute of Nutrition Jun 1996. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT The rate of liver protein synthesis in vivo and hepatic messenger ribonucleic acid (mRNA) levels were measured to examine the mechanisms regulating liver protein synthesis in chicks under various nutritional conditions. Fractional synthesis rate (FSR) of liver protein was measured by using a large dose injection of L-[4-(3)H]phenylalanine. Poly(A)+RNA was extracted as mRNA from total RNA using poly(U)-Sepharose 4B affinity chromatography. The influence of varying dietary protein levels (0-60%) on protein synthesis and mRNA concentration in the liver of chicks was examined. Both FSR (%/d) and absolute synthesis rate (ASR, mg/d) of liver protein increased with increasing dietary ...