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Article: Relationship of protein synthesis to mRNA levels in the muscle of chicks under various nutritional conditions
- Article from:
- The Journal of Nutrition
- Article date:
- July 1, 1996
- Author:
CopyrightCopyright American Institute of Nutrition Jul 1996. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT The relationship between the rate of protein synthesis and the level of messenger RNA (mRNA) in the breast muscle (pectoralis major) was studied to examine the mechanisms regulating muscle protein synthesis in chicks under various nutritional conditions. The fractional synthesis rate (FSR) of protein was measured by a large-dose injection of L-143H]phenylalanine. Poly(U)-Sepharose 4B affinity chromatography was used to extract Poly(A)+ RNA as mRNA. The first study examined the effect of dietary protein levels (0-60%) on protein synthesis rate and the contents of mRNA and total RNA. The fractional synthesis rate increased with increasing dietary protein levels from 0 to 20%. When ...