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Article: pH corrections and protein ionization in water/guanidium chloride
- Article from:
- Biophysical Journal
- Article date:
- December 1, 2001
- Author:
CopyrightCopyright Biophysical Society Dec 2001. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT More than 30 years ago, Nozaki and Tanford reported that the pK values for several amino acids and simple substances in 6 M guanidinium chloride differed little from the corresponding values in low salt (Nozaki, Y., and C. Tanford. 1967. J. Am. Chem. Soc. 89:736-742). This puzzling and counter-intuitive result hinders attempts to understand and predict the proton uptake/release behavior of proteins in guanidinium chloride solutions, behavior which may determine whether the AGN_D values obtained from guanidinium chloride-induced denaturation data can actually be interpreted as the Gibbs energy difference between the native and denatured states (Bolen, D. W., and M. Yang. 2000. ...