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Current status of fluorescent in-situ hybridisation.(materials)
- Article from:
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Medical Device Technology
- Article date:
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May 1, 2004
- Author:
- Jain, K.K.
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Copyright informationCOPYRIGHT 2004 Advanstar Communications, Inc. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Several modifications have taken place in fluorescent in-situ hybridisation (FISH) techniques, including comparative genomic hybridisation, primed in-situ labelling, interphase FISH, multicolour FISH and in combination with peptide nucleic acid technology. FISH can also be combined with microarrays. Selected innovative technologies are described. The most clinically important applications are in cytogenetics and ontology.
Priniciples of FISH
In-situ hybridisation (ISH) has emerged as a powerful and versatile tool for the detection and localisation of nucleic-acid sequences within an intact cell or chromosome, or tissue preparation by means of labelled complementary ...
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